目的 建立锦鸡儿中白桦脂酸,齐墩果酸,熊果酸和羽扇豆醇4种萜类化合物定量分析的高效液相色谱方法,并考察了它们的体外α-葡萄糖苷酶活性。方法 采用Agilent Eclipse XDB-C18色谱柱(4.6 mm×250 mm,5 μm),流动相为甲醇-0.1%磷酸水溶液(85:15),流速为0.6 mL·min-1,柱温25 ℃,检测波长204 nm,对锦鸡儿中的白桦脂酸、齐墩果酸和熊果酸进行含量测定;采用Inert Sustain C18色谱柱(4.6 mm×150 mm,5 μm),流动相为乙腈-水(70:30),流速0.8 mL·min-1,柱温30 ℃,检测波长204 nm,测定锦鸡儿中羽扇豆醇的含量。结果 白桦脂酸、齐墩果酸、熊果酸和羽扇豆醇在0.56~5.58、0.42~4.20、0.19~1.92和6.10~61.00 μg内线性关系良好,平均加样回收率分别为99.79%、98.51%、98.05%和99.47%,RSD分别为1.53%,1.74%,1.78%和1.94%。结论 本试验建立的方法准确可靠,可用于锦鸡儿药材的质量控制;除白桦脂酸外,其他3种化合物均表现出良好的对α-葡萄糖苷酶抑制活性,有望开发成为新的降血糖药物。
Abstract
OBJECTIVE To establish HPLC methods for the determination of betulinic acid, oleanolic acid, ursolic acid, and lupeol in Caragana sinica roots, and investigate the α-glucosidase activity of the four compounds in vitro. METHODS An Agilent Eclipse XDB-C18 column (4.6 mm×250 mm, 5 μm) was applied with methanol-water-phosphoric acid (85:15:0.1) as the mobile phase at a flow rate of 0.6 mL·min-1 to separate betulinic acid, ursolic acid, and oleanolic acid. An Inert Sustain C18 column (4.6 mm×150 mm,5 μm) was applied with acetonitrile-water (70:30) as the mobile phase at a flow rate of 0.8 mL·min-1 to separate lupeol. RESULTS Good linearities were achieved for betulinic acid, oleanolic acid, ursolic acid, and lupeol within the range of 0.56-5.58, 0.42-4.20, 0.19-1.92, and 6.10-61.00 μg, respectively. The average recoveries were 99.79%, 98.51%, 98.05%, and 99.47%, and the RSDs were 1.53%,1.74%,1.78%, and 1.94%, respectively. CONCLUSION The developed methods are accurate and can be used for the quality control of Caragana sinica. In addition to betulinic acid, the other three compounds show good α-glucosidase inhibitory activity and are expected to be developed as new hypoglycemic drugs.
关键词
锦鸡儿 /
三萜类化合物 /
高效液相色谱法 /
含量测定 /
α-葡萄糖苷酶活性
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Key words
Caragana sinica /
triterpene composition /
HPLC /
content determination /
α-glucosidase activity
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中图分类号：
R917
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脚注
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基金
河南省教育厅科学技术研究重点项目(13B360913);河南省高校科技创新团队(16IRTSTHN019); 开封市科技发展计划项目(1503006)
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